Intestinal subepithelial myofibroblasts support the growth of intestinal epithelial stem cells.
Intestinal epithelial stem cells (ISCs) are the focus of recent intense study.Current in vitro models rely on supplementation with the Wnt agonist R-spondin1 to support robust growth, ISC self-renewal, and differentiation.Intestinal subepithelial myofibroblasts (ISEMFs) are important supportive cells within the ISC niche.We hypothesized that co-cul